The main objective of this research work is to investigate the moringa oleifera biodiesel (MOB) as an alternative fuel in a CI engine with hydrogen enriched intake air. The experiment was conducted in a Kirloskar AV1 model single cylinder direct injection diesel engine which produces 3.7 kW power output. MOB was produced by raw Moringa oleifera oil using transesterification process. For hydrogen addition, engine intake manifold was modified. Pure hydrogen was inducted at 4, 6 and 8 lpm (volume basis) in the intake manifold along with air and the experiment was conducted at 20%, 40%, 60%, 80% and 100% load. Test result reveals that direct use of MOB resulted in very high specific fuel consumption (BSFC) and reduces brake thermal efficiency (BTE) of an engine. Exhaust emissions such as CO and HC were reduced significantly with MOB compared to neat diesel fuel, whereas smoke intensity level and NOx increased drastically. Hydrogen enrichment with MOB further decreases the CO and HC emission at all the loads. Also, a significant decrease in smoke intensity level and improvement in BTE was noted with hydrogen addition.